ki-۶۷ expression in hydatidiform moles and hydropic abortions

نویسندگان

alireza khooei department of pathology, imam reza hospital, mashhad university of medical sciences, mashhad, ir iran

fatemeh atabaki pasdar department of anatomical sciences, urmia university of medical sciences, urmia, ir iran; department of anatomical sciences, urmia university of medical sciences, urmia, ir iran. tel: +98-4413444352, fax: 98-4412780801

alireza fazel department of anatomy and cell biology, mashhad university of medical sciences, mashhad, ir iran

mahmoud mahmoudi immunology research center, bu ali research institute, mashhad university of medical sciences, mashhad, ir iran

چکیده

conclusions despite the significant differences , ki-67 immunostaining could not be helpful in distinguishing molar placentas from hydropic abortions as well as partial from complete hydatidiform moles, because there are considerable overlaps between results in different categories. objectives this study was undertaken to determine whether the expression of ki-67 protein could differentiate these entities. materials and methods we performed ki-67 immunohistochemical staining in 19 molar (8 partial and 11 complete moles) and 10 non-molar (hydropic abortions) formalin-fixed, paraffin-embedded tissue samples. ploidy analysis using flow cytometry had confirmed diploidy in hydropic abortions and complete moles and triploidy in partial moles. results ki-67 immunoreactivity was assessed in villous cytotrophoblasts, syncytiotrophoblasts and stromal cells. positive cells were found to be restricted mostly to the villous cytotrophoblasts, while syncytiotrophoblasts showed an absence of immunostaining for ki-67, and occasional weak nuclear staining was seen in the stromal cells. there was a significant difference in ki-67 immunoreactivity of cytotrophoblastic cells between hydropic abortions and complete moles (p < 0.001), hydropic abortions and partial moles (p = 0.002) and also between complete and partial moles (p < 0.001). on the other hand, there is significant overlap in the ki-67 immunoreactivity between complete and partial moles (++ staining category) and between partial moles and hydropic abortions (+ staining category). background differential diagnosis of hydatidiform moles from non-molar specimens as well as their sub-classification such as complete and partial hydatidiform moles are important for clinical management and accurate risk assessment for persistent gestational trophoblastic disease, but diagnosis based solely on histomorphology suffers from poor interobserver and intraobserver reproducibility.

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منابع مشابه

Ki-67 Expression in Hydatidiform Moles and Hydropic Abortions

BACKGROUND Differential diagnosis of hydatidiform moles from non-molar specimens as well as their sub-classification such as complete and partial hydatidiform moles are important for clinical management and accurate risk assessment for persistent gestational trophoblastic disease, but diagnosis based solely on histomorphology suffers from poor interobserver and intraobserver reproducibility. ...

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Use of proliferation cell nuclear antigen immunoreactivity for distinguishing hydropic abortions from partial hydatidiform moles.

AIMS To determine whether the expression of proliferating cell nuclear antigen (PCNA) in villous cytotrophoblast could distinguish between placental tissue from a hydropic abortion and that from a partial hydatidiform mole. METHODS Tissue from 18 partial hydatidiform moles, 15 hydropic abortions, five normal first trimester placentas and five normal full term placentas were immunostained for ...

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Flow Cytometric DNA Analysis and Histopathologic Re-Evaluation of Paraffin Embedded Samples from Hydatidiform Moles and Hydropic Abortions

Background Distinction of hydatidiform moles (HMs) from non-molar abortions and sub-classification of HMs are important for clinical practice; yet, diagnosis based solely on morphology is affected by interobserver variability. The objective of this study was to determine the role of DNA flow cytometry in distinguishing molar from non-molar pregnancies. MaterialsAndMethods This retrospective stu...

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Flow Cytometric DNA Analysis and Histopathologic Re-Evaluation of Paraffin Embedded Samples from Hydatidiform Moles and Hydropic Abortions

BACKGROUND Distinction of hydatidiform moles (HMs) from non-molar abortions and sub-classification of HMs are important for clinical practice; yet, diagnosis based solely on morphology is affected by interobserver variability. The objective of this study was to determine the role of DNA flow cytometry in distinguishing molar from non-molar pregnancies. MATERIALS AND METHODS This retrospective...

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Tetraploidy in hydatidiform moles.

STUDY QUESTION How does tetraploidy develop in hydatidiform moles (HMs), and what is the frequency of the different origins? SUMMARY ANSWER Most molar pregnancies with tetraploid cells appear to be produced by somatic endoreduplications, while a minority originate from a tetraploid zygote. The frequency of zygotic tetraploidy was estimated to be 0.7%. WHAT IS KNOWN ALREADY The parental orig...

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flow cytometric dna analysis and histopathologic re-evaluation of paraffin embedded samples from hydatidiform moles and hydropic abortions

background: distinction of hydatidiform moles (hms) from non-molar abortions and sub-classification of hms are important for clinical practice; yet, diagnosis based solely on morphology is affected by interobserver variability. the objective of this study was to determine the role of dna flow cytometry in distinguishing molar from non-molar pregnancies. materials and methods: this retrospective...

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عنوان ژورنال:
iranian red crescent medical journal

جلد ۱۵، شماره ۷، صفحات ۵۹۰-۵۹۴

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